The 5th FLxFlow (Lisbon Flow Cytometry Network) course will introduce the basic principles and applications of flow cytometry to beginners and advanced users. From fluorophores and panel design, instruments operation, required controls and statistical analysis to data publication and potential applications. The course will consist of theoretical talks and  theoretical practical sessions where students will learn how to set up an experiment, run a multi-color experiment, perform compensation & spectral unmixing and analyze data. There will also be practical hands-on sessions in immunophenotyping by conventional and spectral flow cytometry, cell sorting and imaging flow for selected students (selection based on course application letter).

Advanced courses are offered as part of the PhDCAML Doctoral Program to doctoral students at Lisbon School of Medicine, which allow our students to enrich their scientific careers. External students can also apply.